Patent Number: 8,822,670

Title: Method of synthesizing cDNA and method of synthesizing RNA chains, and nucleotide-immobilized carrier

Abstract: A method of synthesizing a cDNA chain using an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a group derived from a phosphoric ester composing the hydrophilic portion of phospholipid, and a second unit having a group derived from carboxylic acid having an electron-attractive substituent bound to a carbonyl group, which includes immobilizing a polynucleotide for DNA elongation; bringing a solution containing an RNA fragment, nucleotide monomers, and a reverse transcriptase or an enzyme having polymerase activity into contact with the surface of the insoluble carrier; and allowing the polynucleotide for DNA elongation immobilized on the surface of the carrier to elongate using the RNA fragment contained in the solution as a template, to thereby form a single-strand cDNA.

Inventors: Kinoshita; Kenji (Hyogo, JP), Yakabe; Toru (Tokyo, JP), Fujimoto; Kentaro (Tokyo, JP), Yokoyama; Kanehisa (Tokyo, JP), Fujiwara; Kazuhiko (Tokyo, JP)

Assignee: Sumitomo Bakelite Company, Ltd.

International Classification: C07H 21/00 (20060101)

Expiration Date: 9/02/12018