Patent Number: 8,871,435

Title: Methods and compositions for identifying agents that inhibit an NS4B-mediated neoplastic cellular phenotype of HCV infected cells

Abstract: Methods and compositions for identifying agents that inhibit a neoplastic cellular phenotype mediated by the NS4B protein nucleotide binding motif (NBM) of hepatitis C virus (HCV) are provided. In general, the methods involve contacting a candidate agent with a mammalian cell expressing an NS4B NBM polypeptide of an HCV virus, wherein expression of the NS4B NBM polypeptide in the absence of candidate agent promotes a neoplastic cellular phenotype, and detecting the presence or absence of an effect of the candidate agent on NS4B-mediated promotion of a neoplastic cellular phenotype. The provided methods and compositions find use in a variety of therapeutic and screening applications.

Inventors: Glenn; Jeffrey S. (Palo Alto, CA), Einav; Shirit (Stanford, CA)

Assignee: The Board of Trustees of the Leland Stanford Junior University

International Classification: C12Q 1/02 (20060101); C12Q 1/70 (20060101); C12N 5/00 (20060101); C12Q 1/18 (20060101)

Expiration Date: 2018-10-28 0:00:00