Patent Number: 8,877,463

Title: Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase, and use thereof

Abstract: The present invention relates to Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase and use thereof. The polypeptide has an activity of converting PPD (protopanaxadiol)-type saponins into in vivo absorbable and highly active deglycosylated saponins, by selective hydrolysis of a bond at a particular position of ginsenoside. The present invention also relates an amino acid sequence constituting the polypeptide, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, and a transformant transformed with the vector. The invention further provides a method for preparing Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase by culturing the transformant, a method for converting PPD (protopanaxadiol)-type major ginsenoside into a rare ginsenoside that is absorbable in vivo using the protein, and a composition for converting PPD-type saponins into in vivo absorbable saponins, having the protein as an active ingredient.

Inventors: An; Dong-Shan (Daejeon, KR), Kim; Song-Gun (Daejeon, KR), Lee; Sung-Taik (Daejeon, KR), Im; Wan-Taek (Daejeon, KR), Cui; Chang-Hao (Daejeon, KR)

Assignee: Korea Research Institute of Bioscience and Biotechnology

International Classification: C12P 19/56 (20060101); C12N 15/70 (20060101); C12N 15/63 (20060101); C12N 15/56 (20060101); C12N 1/21 (20060101); C12N 9/24 (20060101); A61K 31/704 (20060101)

Expiration Date: 2019-11-04 0:00:00