Patent Number: RE37,002

Title: Lipid-dispersed solution, production thereof and use in determining lipid levels

Abstract: .[.There is disclosed an aqueous dispersed solution as a standard solution for determining lipid levels, having a dispersion form and a particle size, which are similar to those of serum lipids. A lipid-dispersed solution is obtained by evaporating an organic solvent from a mixture prepared by adding cholesterol, a phospholipid, a bile acid or bile acid salt, and a neutral lipid and/or a cholesterol ester in the organic solvent, swelling the resultant thin-film like mixture with water or buffer heated at a temperature higher than a phase transition temperature of the lecithin, and then dispersing the solution by a physical shear force. There can be easily obtained a solution having a cholesterol concentration of about less than 1,000 mg/dl, which is extremely stable and can be stored for a long period of time..]. .Iadd.There is disclosed an aqueous dispersed solution as a standard solution for determining lipid levels, having a dispersion form and a particle size, which are similar to those of serum lipids. A lipid-dispersed solution is obtained by evaporating an organic solvent from a mixture prepared by adding cholesterol, a phospholipid, a bile acid or bile acid salt, and a neutral lipid and/or a cholesterol ester in the organic solvent, swelling the resultant thin-film like mixture with water or buffer heated at a temperature higher than a phase transition temperature of the lecithin, and then dispersing the solution by a physical shear force. These can be easily obtained a solution having a cholesterol concentration of about less than 1,000 mg/dl, which is extremely stable and can be stored for a long period of time. The lipid dispersed solution is used as a standard solution in calibrating a device for performing enzymatic lipid level determination, and the calibrated device is used to determine the lipid level (e.g. cholesterol) in a solution.

Inventors: Yamamoto; Tomohiro (Neyagawa, JP), Yoshioka; Toshihiko (Osaka, JP), Nankai; Shiro (Hirakata, JP)

Assignee: Matsushita Electric Industrial Co., Ltd.

International Classification: G01N 33/96 (20060101); G01N 33/92 (20060101); B01J 013/00 (); C12Q 001/60 ()

Expiration Date: 12/26/2017